F(ab')2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC/F(ab')2-羊抗大鼠IgG (H+L) 交叉吸附, FITC

货号：PA5-33282

规格：500 µL

价格：3959

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：山羊

抗体亚型：IgG

荧光染料：FITC

类型:	二抗	同型对照：	IgG
浓度：	0.7 mg/mL	用法：	1:25-1:100(Flow);2.0 µg/mL(ICC);2.0 µg/mL(IF)

产品详细信息PA5-33282 targets Rat IgG in FACS applications. Cross-reactivity with mouse IgG has been reduced by adsorption.For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (PA5-33282) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Rat IgG Cross Adsorbed Secondary Antibody, FITC was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperature. F (ab')2-Goat anti-Rat IgG Cross Adsorbed Secondary Antibody, FITC (Product # PA5-33282) was used at a concentration of 2µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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