CD9 Recombinant Rabbit Monoclonal Antibody/CD9 重组兔单抗

货号：MA5-33125

规格：100 µL

价格：5840

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Synthesized CD9

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：	IgG
浓度：	0.225 mg/mL	用法：	1:30-1:200(ICC);1:30-1:200(IF);1:30-1:200(IHC);1:500-1:5000(WB)

靶标信息CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

数据

CD9 Antibody (MA5-33125) in IFImmunofluorescent analysis of CD9 in MCF-7 cells using a CD9 monoclonal antibody (Product # MA5-33125) at a dilution of 1:34. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI. CD9 Antibody (MA5-33125) in IHC (P)Immunohistochemical analysis of CD9 in paraffin embedded human small intestine tissue using a CD9 monoclonal antibody (Product # MA5-33125) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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