Mouse anti-Human Kappa Light Chain Secondary Antibody/鼠抗人κ轻链二抗

货号：MA5-12117

规格：500 µL

价格：4034

产品类型：二抗

品牌：Thermo Fisher

抗原：B lymphoma cells

物种：人

宿主：小鼠

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	酶联免疫吸附实验 (ELISA) 1 µg/mL 流式细胞分析 (Flow) 1 µg/mL 免疫组化（石蜡) (IHC (P)) 1:400-800 免疫印迹 (WB) 1:400-1:1600

By Western blot, MA5-12117 detects Human Ig kappa light chain at ~25kD in human samples.By ELISA and WB, MA5-12117 does not react with human Ig lambda light chain.靶标信息Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Figure 1.Human Kappa Light Chain Secondary Antibody (MA5-12117) in IHCFormalin-fixed, paraffin-embedded human tonsil stained with Kappa Light Chain antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of B lymphocytes. Figure 2.Human Kappa Light Chain Secondary Antibody (MA5-12117) in WBWestern blot analysis of immunoglobulin (Ig) kappa light chain was performed by loading 25 µg of Raji (IgG/kappa-expressing B cells), Ramos (IgG/lambda-expressing B cells), and Jurkat (T cells) whole cell lysates, 0.25 µg of purified human IgG/kappa and human IgG/lambda, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Ig kappa light chain was detected at ~25 kD using a mouse anti-human Ig kappa light chain monoclonal antibody (Product # MA5-12117) diluted to a concentration of 0.5 µg/mL in blocking buffer, overnight at 4C overnight on a rocking platform. The blot was washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

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参考文献：

1.Cancer cytopathologyRole of Epstein-Barr virus status and immunophenotypic studies in the evaluation of exfoliative cytology specimens from patients with post-transplant lymphoproliferative disorders.2.Diagnostic cytopathologyLiquid-based cytologic findings of solitary extramedullary plasmacytoma in thyroid: a case report identified with fine-needle aspiration cytology.3.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, IncThyroid carcinoma-associated genetic mutations also occur in thyroid lymphomas. 4.International journal of cancer Suppression of human melanoma tumor growth in SCID mice by a human high molecular weight-melanoma associated antigen (HMW-MAA) specific monoclonal antibody. 5.Diagnostic pathology Composite lymphoma in the anterior mediastinum: a case report and review of the literature.

技术参数

产品应用 ELISA;Flow;IHC (P);WB;ICC;IHC;Neu

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