Rabbit anti-Human IgG (Heavy chain) Recombinant Secondary Antibody/兔抗人IgG（重链）重组二抗

货号：SA5-10223

规格：100 µg

价格：2732

产品类型：二抗

品牌：Thermo Fisher

抗原：Peptide corresponding to Human IgG.

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	50-200 ng/well (Capture), 0.05-0.2 µg/mL (Detect)(ELISA)；0.5-2 µg/mL(ICC)；0.5-2 µg/mL（IF）；0.5-2 µg/mL（IHC）

产品详细信息This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, 2, 3, and 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgGRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据：

Human IgG (Heavy chain) Secondary Antibody (SA5-10223) in IHC Immunohistochemistry of Human Tonsil using Anti-Gamma Heavy Chain antibody Rabbit Anti-Human IgG, Heavy Chain Secondary Antibody (Product # SA5-10223). Human IgG (Heavy chain) Secondary Antibody (SA5-10223) in ELISACross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of Rabbit anti-Human IgG (Heavy chain) Secondary Antibody (Product # SA5-10223), Rabbit anti-Human IgG1 Secondary Antibody (Product # SA5-10202), Rabbit anti-Human IgG2 Secondary Antibody (Product # SA5-10203), Rabbit anti-Human IgG3 Secondary Antibody (Product # SA5-10204), Rabbit anti-Human IgG4 Secondary Antibody (Product # SA5-10205), Rabbit anti-Human Kappa Light Chain Secondary Antibody (Product # SA5-10208), and Rabbit anti-Human Lambda Light Chain Secondary Antibody (Product # SA5-10209) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. Human IgG (Heavy chain) Secondary Antibody (SA5-10223) in ELISAELISA of human immunoglobulins shows Rabbit Anti-Human IgG, Heavy Chain Secondary Antibody (Product # SA5-10223) reacts to the 1, 2, 3, 4 heavy chains of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of Rabbit Anti-Human IgG, Heavy Chain Secondary Antibody (Product # SA5-10223) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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技术参数

产品应用 ELISA;ICC;IF;IHC

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