Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red-X/s山羊抗小鼠IgG (H+L)交叉吸附二抗，Texas Red-X

货号：T-6390

规格：1 mg

价格：2649

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Texas Red

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	2 µg/mL (ICC)； 2 µg/mL (IF);Assay Dependent(IHC)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (T-6390) in IF Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red®-X conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red®-X (Product # T-6390) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (T-6390) in IF Confocal micrograph illustrating sexual reproduction of the ciliate protist, Tetrahymena thermophila, six hours after mating. After fixation and permeabilization, the cytoskeleton was labeled with an anti-tubulin antibody and subsequently visualized with Texas Red®-X goat anti-mouse IgG antibody (Product # T-6390). The macro- and micronuclei were stained with SYTOX® Green nucleic acid stain (Product # S7020). Image contributed by David Asai and Amy Walanski, Purdue University. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (T-6390) in IFChinook salmon embryo (CHSE) cells infected with hematopoietic necrosis virus. Cell nuclei were stained with DAPI (Product # D1306, D3571), and virions were labeled with mouse monoclonal IgG directed against viral matrix protein and Texas Red®-X goat anti-mouse IgG (Product # T-6390). The image was acquired with a Leica TCS 4D confocal laser scanning microscope. Image contributed by C.H. Kim, Oregon State University.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Molecular medicine reportsDifferential long non‑coding RNA and mRNA expression in differentiated human glioblastoma stem cells.2. Eukaryotic cellA contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.3. PloS oneHeterogeneity in macrophage phagocytosis of Staphylococcus aureus strains: high-throughput scanning cytometry-based analysis.4. CellThe molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain.5. Chemistry and biologySynthetic compound libraries displayed on the surface of encoded bacteriophage.

技术参数

产品应用 ICC;IF;IHC;Misc

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21630.htm