Donkey anti-Mouse IgG (H+L) ReadyProbes™ Secondary Antibody, Alexa Fluor 488/驴抗小鼠IgG (H+L) ReadyProbes™二抗， Alexa Fluor 488

货号：R37114

规格：2 x 2.5 mL

价格：1768

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobin

物种：小鼠

宿主：驴

抗体亚型：IgG

荧光染料：Alexa Fluor 488

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：		用法：	Assay-Dependent（Flow）； Assay-Dependent (ICC)； Assay-Dependent (IF);1:1000 (IHC(F))

产品详细信息No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins. Like other ReadyProbes reagents, Alexa Fluor 488 Donkey anti-Mouse IgG Antibody comes in a convenient dropper bottle.靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

数据

Mouse IgG (H+L) Secondary Antibody (R37114) in IHC (F)Immunofluorescent analysis of beta-3 Tubulin (green) and PAX6 (red) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Product # MA1-118) at a dilution of 1:500, and a Rabbit PAX6 polyclonal antibody (red; Product # 42-6600) at a dilution of 1:500 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114) and Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042) at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University. Mouse IgG (H+L) Secondary Antibody (R37114) in IFImmunofluorescence analysis of Donkey anti-Mouse IgG Secondary Antibody, Alexa Fluor 488 was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Donkey anti-Mouse IgG Secondary Antibody, Alexa Fluor 488 (Product # R37114) was used at 1:5000 dilution in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of Cytokeratin 19 in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Secondary Antibody (R37114) in IHC (F)Immunofluorescent analysis of beta-3 Tubulin (green), PAX6 (red), and CTIP2 (blue) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Product # MA1-118) at a dilution of 1:500, a Rabbit PAX6 polyclonal antibody (red; Product # 42-6600) at a dilution of 1:500, and a Rat CTIP2 polyclonal antibody (blue) at a dilution of 1:300 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114), Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042), and Goat anti-Rat Alexa Fluor 350 (Product # A-21093) at a dilution of 1:1000 in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Data courtesy of Dr. Zhexing Wen at Emory University.

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参考文献：

1. eNeuroWestern High-Fat Diet Consumption during Adolescence Increases Susceptibility to Traumatic Stress while Selectively Disrupting Hippocampal and Ventricular Volumes.2.Human molecular geneticsMutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.3. Molecular medicine reportsDirected differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells.4. Nature communicationsLncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.5. Nature immunologyiRhom2 is essential for innate immunity to DNA viruses by mediating trafficking and stability of the adaptor STING.

技术参数

产品应用 Flow;ICC;IF;IHC(F);WB;IHC

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