Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP/山羊抗小鼠IgG (H+L)交叉吸附二抗，AP

货号：G-21060

规格：1 mg

价格：2335

产品类型：二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1:500-1:2,000（ELISA）； 1:500-1:2,000 (WB)； 1:500-1:2,000 (IHC)

产品详细信息

The specific activity of the AP conjugates is approximately 150–350 units/mg. One unit is defined as the amount of enzymatic activity required to hydrolyze 1.0 micromole of p nitrophenyl phosphate to p-nitrophenol and inorganic phosphate in 60 seconds at 21°C, pH 10.Reconstituted using 0.5 mL of deionized water to make a 2 mg/mL stock solution.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (G-21060) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and MCF7 (Lane 2). The blots were probed with Anti-GLO1 Mouse Monoclonal Antibody (Product# MA1-13029, 1 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP conjugate (Product # G-21060) at dilutions 1:500 (Fig. 1) and 1:2,000 (Fig. 2). A 21 kDa band corresponding to GLO1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product #WP20003). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (G-21060) in WB Immunodetection on a Western blot with the BOLD APB chemiluminescent substrate. Samples of protein molecular weight standards (Product # P-6649) containing decreasing amounts of a-tubulin were run on an SDS-polyacrylamide gel and blotted onto a PVDF membrane. After electrophoresis, the blot was stained with SYPRO® Ruby protein blot stain (Product # S-11791) to detect total protein. After documentation of the total protein stain (top), the blot was incubated with a mouse monoclonal anti-a-tubulin antibody (Product # A11126), followed by an alkaline phosphatase conjugate of goat anti-mouse IgG antibody (Product # G-21060). Finally, the blot was stained with the BOLD APB chemiluminescent substrate (Product # B21901) to detect the alkaline phosphatase enzyme. The chemiluminescent signal was visualized using a scanner in chemiluminescence detection mode.

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参考文献：

1. Parasitology researchInduction of specific humoral immune response in mice immunized with ROP18 nanospheres from Toxoplasma gondii.2. The Journal of biological chemistryMAHRP-1, a novel Plasmodium falciparum histidine-rich protein, binds ferriprotoporphyrin IX and localizes to the Maurer's clefts.3. ProteomicsSimultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates.4. ProteomicsSimultaneous red/green dual fluorescence detection on electroblots using BODIPY TR-X succinimidyl ester and ELF 39 phosphate.5. The Journal of cell biologyStructural requirements for localization and activation of protein kinase C mu (PKC mu) at the Golgi compartment.

技术参数

产品应用 ELISA;IHC;WB;Misc

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