Rabbit anti-Mouse IgG (H+L), Recombinant Secondary Antibody, HRP/兔抗小鼠IgG（H + L），重组二级抗体，HRP

货号：A27025

规格：1 mg

价格：2547

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：HRP

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.05-1 µg/mL(WB)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG (H+L) Secondary Antibody (A27025) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected using Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27025) at concentrations 1 µg/mL (Fig. 1), 0.01 µg/mL (Fig. 2) and 0.05 µg/mL (Fig. 3). A 24 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106) Mouse IgG (H+L) Secondary Antibody (A27025) in WBExpression of endogenous GAPDH (panel a) and alpha-Tubulin (panel b) was detected using the respective Mouse primary antibody and Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27025) (0.4 µg/mL, 1:2500 dilution). Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa human cervical carcinoma cells (lane 1-3) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System Chemiluminescence was detected using the Pierce™ ECL Western Blotting Substrate (Product # 32106)

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