Rabbit anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody/兔抗小鼠IgG (H+L)Superclonal™重组二抗

货号：A27022

规格：1 mg

价格：1915

产品类型：二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.01-0.5 µg/mL (ELISA);0.05-1 µg/mL (WB)；0.1-1 µg/mL (ICC)；0.1-1 µg/mL (IF)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG (H+L) Secondary Antibody (A27022) in IFalpha-Tubulin (green) in U20S human osteocarcinoma cells was labeled with Mouse anti-alpha-Tubulin primary antibody (Product # 32-2500) (1:3000), Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A27022) (0.4 µg/mL) and visualized using Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) as tertiary antibody (Product # A-11008) (1:400). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). F-actin (red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381) (1:200). Representative images with 3 lots of Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A27022) for lot-to-lot consistency of performance (panel a-c) and no primary control (panel d) to assess background are shown. Mouse IgG (H+L) Secondary Antibody (A27022) in WB Expression of endogenous alpha-Tubulin was detected at 50 KDa using Mouse anti-alpha-Tubulin primary antibody (Product # 32-2500) (1:3000) and Rabbit anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Unconjugated (Product # A27022) (0.4 µg/mL, 1:2500 dilution). Goat anti-Rabbit IgG (H+L), HRP conjugate as the tertiary Antibody (G21234) (1:3000).Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa human cervical carcinoma cells (lane 1-3) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. Chemiluminescence was detected using the Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (A27022) in ELISA ELISA was performed using purified IgG from different species to determine the cross-reactivity and lot-to-lot consistency of Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A27022). Goat IgG (GT003), Mouse IgG (Product # 10400C), Rat IgG (Product # 10700), Human IgG (Product # 12000C), Bovine IgG (Bo003), Guinea Pig IgG (Gp003) and Donkey IgG (Dk-003-B) were coated onto the plate (coating conc 0.02 µg/mL) Three point dilution of Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A27022) was used to determine the cross-reactivity of three lots around the EC50 (25 ng/mL) of binding to the target antigen (Mouse IgG). Goat anti-Rabbit HRP (Product # A16104, 1:5000) was used as the detector antibody.

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