Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 546/山羊抗小鼠IgG2a交叉吸附二抗，Alexa Fluor 546

货号：A-21133

规格：500 µg

价格：3033

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG2a

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 546

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL（Flow）； 4 µg/mL（ICC）; 4 µg/mL (IF); 1-10 µg/mL (IHC)

点击查看所有Alexa Fluor 荧光二抗靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21133) in IFImmunofluorescence analysis of Goat anti-Mouse IgG2a Secondary Antibody, Alexa Fluor 546 was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Goat anti-Mouse IgG2a Secondary Antibody, Alexa Fluor 546 (Product # A-21133) was used at a concentration of 4µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of Cytokeratin 19 in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 633 Phalloidin A22284, 1:300) (Panel c: green; pseudo color). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Neuropathology and applied neurobiologyInvestigating complex I deficiency in Purkinje cells and synapses in patients with mitochondrial disease.2. Clinical cancer research : an official journal of the American Association for Cancer ResearchChromosome 5q loss in colorectal flat adenomas.3. Investigative ophthalmology and visual scienceExpression and localization of neural cell adhesion molecule and polysialic acid during chick corneal development.4.Biology of reproductionLoss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.5.The Journal of biological chemistryDual specificity A-kinase anchoring proteins (AKAPs) contain an additional binding region that enhances targeting of protein kinase A type I.

技术参数

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