Rat anti-Mouse IgG2a Secondary Antibody, Biotin, eBioscience/大鼠抗小鼠IgG2a二抗，Biotin

货号：13-4210-82,13-4210-80

规格：100 µg,25 µg

价格：2217,1011

产品类型：荧光二抗

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：m2a-15F8

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	0.5 mg/mL	用法：	10 µg/mL(ICC)；10 µg/mL(IF)；10 µg/mL(IHC (P))

产品详细信息Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.Applications Tested: This m2a-15F8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue and by immunocytochemistry on fixed MDCK cells. The antibody can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG2a Secondary Antibody (13-4210-82) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL Mouse IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human CD20 Purified (Product # 14-0202-82) (right) followed by 10 µg/mL Anti-Mouse IgG2a Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.

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