| 产品介绍 | | |
| Rat anti Mouse THY-1.2 antibody, clone FF-10recognizes the murine Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.Rat anti Mouse THY-1.2 antibody, clone FF-10 strongly induces homotypic adhesion of Thy1.2 positive cell lines. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Immunogen | EL-4 cells. |
| Approx. Protein Concentrations | IgG concentration 4 mg/ml |
| Fusion Partners | Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | |
| Western Blotting | √ | | |
| Immunoprecipitation | √ | | |
| Flow Cytometry | √ | 1/50 | 1/100 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western BlottingMCA1474 recognises a 30kD glycoprotein in immunoprecipitation and Western blotting assays.
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| 文献 |
1. Fan, X.et al. (1996) ICAM-1, -2, and LFA-1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule.Inflammation 20: 401.2. Venugopalan Pet al.(2016) Transplanted neurons integrate into adult retinas and respond to light.Nat Commun. 7: 10472.3. Szczerbal, I.et al.(2009) The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system.Chromosoma. 118 (5): 647-63.4. Catignas, K.K.et al.(2021) αVintegrins in Schwann cells promote attachment to axons, but are dispensablein vivo..Glia. 69 (1): 91-108.
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