Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, FITC/山羊抗小鼠IgG2a交叉吸附二抗，FITC

货号：31634

规格：500 µg

价格：4053

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG2a, kappa

荧光染料：FITC

类型:	二抗	同型对照：
浓度：	1.6 mg/mL	用法：	1 µg/mL(ICC)；1 µg/mL(IF)；1:50 - 1:200(IHC)；1:50 - 1:200(IP)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31634 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31634 reacts with the heavy chains of mouse IgG2a, but not with heavy chains of other mouse IgG subclasses or with light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 7.6 µg/mg; ~ 3 moles FITC per mole IgG (lot-dependent).Reconstitute with 0.35 mL of distilled water (1.7 mg/mL after restoration).Country of Origin: USA靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG2a Cross-Adsorbed Secondary Antibody (31634) in IFImmunofluorescence analysis of Goat anti-Mouse IgG2a Cross Adsorbed Secondary Antibody, FITC conjugate was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Goat anti-Mouse IgG2a Cross Adsorbed Secondary Antibody, FITC conjugate (Product # 31634) was used at a concentration of 1 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of cytokeratin 19 in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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