Rat anti-Mouse IgG2b Secondary Antibody, FITC, eBioscience™/大鼠抗小鼠IgG2b二抗，FITC, eBioscience™

货号：11-4220-82,11-4220-80

规格：100μg,25μg

价格：2546,1124

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：m2b-25G4

荧光染料：FITC

抗体类型:	荧光二抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow);20 µg/mL (ICC);20 µg/mL (IF);Assay-Dependent (IHC); Assay-Dependent (IHC(P))

产品详细信息Description: The monoclonal antibody m2b-25G4 recognizes Mouse IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. This m2b-25G4 antibody does not recognize other mouse isotypes nor does it crossreact to rat IgG2b or any rat isotype antibodies.Applications Reported: This m2b-25G4 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This m2b-25G4 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG2b primary antibody. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This m2b-25G4 antibody has also been tested by immunocytochemistry of fixed and permeabilized cells stained with a mouse IgG2b primary antibody. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG2b Secondary Antibody (11-4220-82) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (blue histogram) or Anti-Human CD4 Purified (Product # 14-0048-82) (a mouse IgG2b antibody) (purple histogram) followed by 0.06 µg of Anti-Mouse IgG2b FITC. Cells in the lymphocyte gate were used for analysis. Mouse IgG2b Secondary Antibody (11-4220-82) in IHC (P) Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 2.5 µg/mL of Anti-Human CD4 Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.

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参考文献：

1.Frontiers in molecular neuroscienceIncreased White Matter Inflammation in Aging- and Alzheimer's Disease Brain.2.Cellular immunologyIn vivo two-photon imaging of T cell motility in joint-draining lymph nodes in a mouse model of rheumatoid arthritis.3.Experimental and molecular pathologyFLT-3 expression and function on microglia in multiple sclerosis.

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