F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, eFluor 570, eBioscience/F（ab'）2-山羊抗小鼠IgG（H + L）二抗，eFluor 570

货号：41-4010-82,41-4010-80

规格：100 µg ,25 µg

价格：2207,815

产品类型：荧光二抗

品牌：eBioscience

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	10 µg/mL(ICC)；10 µg/mL(IF)；Assay-Dependent(IHC)

产品详细信息Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).Applications Reported: This polyclonal antibody has been reported for use in immunohistochemical staining, and immunocytochemistry.Applications Tested: This polyclonal F(ab')2 Anti-Mouse IgG antibody has been tested by immunocytochemistry after staining with a primary antibody of the mouse isotype. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (41-4010-82) in ICCImmunocytochemistry of fixed and permeabilized HeLa cells using either 1 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 1 µg/mL of Anti-alpha Tubulin Purified (Product # 14-4502-82) (right), followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570. Nuclei are stained with DAPI

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21784.htm