F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot 605/F(ab')2-山羊抗小鼠IgG (H+L)二抗， Qdot 605

货号：Q11002MP,Q-11001MP

规格：100μg,200μL

价格：3235,6836

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	荧光二抗	同型对照：
浓度：		用法：	1:50 (Flow);1:50 (ICC); 1:50 (IF); 1:50 (IHC); 1:50 (WB)

产品详细信息Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Secondary Antibody (Q11002MP) in IF Human Umbilical Vein Endothelial Cells (HUVEC; C0035C) were grown to confluency in Medium 200 (Product # M-200-500) plus Large Vessel Endothelial Supplement (LVES; Product # A1460801). Cells were fixed with 4% formaldehyde and permeablized with 0.25% Triton™ X-100 followed by staining with mouse anti-ATP synthase subunit IF-1 monoclonal antibody (Product # A-21355) and rabbit anti-ß-Catenin polyclonal antibody (Product # 71-2700). Primary antibodies were detected with Qdot® 605 Goat F (ab')2 anti-mouse IgG Conjugate (Red; Product # Q11002MP) and Qdot® 655 Goat F (ab')2 anti-rabbit IgG Conjugate (Magenta, Product # Q-11421MP), respectively. Cells were counterstained with NucBlue® Fixed Cell (Blue; Product # R37606) and ActinGreen™ 488 (Green, Product # R37110) ReadyProbes™ Reagents. Cover slips were air dried, mounted with Cytoseal™ 60, and imaged on a Zeiss LSM 710 confocal microscope.

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参考文献：

1.ACS nanoMolecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots.2.Nano lettersHigh throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals.3.Journal of virologyActivation of Kaposi's sarcoma-associated herpesvirus lytic gene expression during epithelial differentiation.

技术参数

产品应用 IF;IHC;Flow;ICC;WB;Misc

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