Rabbit anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Biotin/兔抗小鼠IgG (H+L), Superclonal™重组二抗，生物素

货号：A27024

规格：1 mg

价格：2295

产品类型：二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent (ELISA)；0.05-1 µg/mL (WB)

产品详细信息

The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据：

Mouse IgG (H+L) Secondary Antibody (A27024) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.25 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Biotin conjugate (Product # A27024) at dilutions 0.05 µg/mL (Fig. 1), 0.5 µg/mL (Fig. 2) and 1 µg/mL (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (A27024) in WB Expression of endogenous Actin (panel a) and alpha-Tubulin (panel b) was detected using the respective Mouse primary antibody and Rabbit anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Biotin conjugate (Product # A27024) (0.4 µg/mL, 1:2500 dilution) with Strep-HRP (Product # SA100-01) as the detector. Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa human cervical carcinoma cells (lane 1-3) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. Chemiluminescence was detected using the Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (A27024) in ELISASandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100 µL of an Apo A-1 Recombinant Rabbit Polyclonal Antibody (Product # 710263) at a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382) overnight at 4C. Wells were blocked with 150 µL of StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for 30 minutes, and 80 µL of recombinant human Apo A-1 (Product # 10686-H02H-50) standards ranging from 1.6-1000 ng/mL (A) or 100 µL of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST (Product # 28360), and 100 µL per well of an Apo A-1 Mouse Monoclonal Antibody (Product # MA5-14667) was added to each well for 1 hour at room temperature. The plate was washed, and 100 µL per well of a biotinylated Rabbit anti-Mouse IgG Recombinant Superclonal™ Secondary Antibody (Product # A27024) was incubated for 1 hour, followed by Streptavidin-HRP (Product # 21126) for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028), followed by Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

产品应用 ELISA;WB

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21840.htm