Rabbit anti IRGM (Internal Region) antibody
货号：AHP1887
规格：0.1 mg
价格：5160
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： IRGM (Internal Region)
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti IRGM (Internal Region) antibodyrecognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47.IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy.IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | Synthetic peptide corresponding to a 14 aa sequence from near the centre of human IRGM. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | | 200ug/ml |
| Immunohistology - Paraffin1 | √ | 5ug/ml | |
| Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human brain
- Western Blotting
- AHP1887 detects a band of approximately 32 kDa in human brain cell lysate.
|
| 数据 |
| Western blot analysis of human brain lysate probed with Rabbit anti Human IRGM (AHP1887) at 1(A) and 2(B) ug/ml.Immunohistochemical staining of human brain with Rabbit anti Human IRGM (AHP1887). |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| Antigen Retrieval Buffer, pH8.0 | BUF025A | IHC(P) | 500 mL |
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
技术参数 产品应用 IF;P*;WB
产品类型 Polyclonal Antibody
推荐用于 IF;P*;WB
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