Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800/山羊抗小鼠IgG (H+L)交叉吸附二抗，DyLight 800

货号：SA5-10176

规格：500 µg

价格：2335

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG-heavy and light chain

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：DyLight 800

抗体类型:	荧光二抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:50-1:2,000(ICC);1:50-1:2,000（IF);1:50-1:2,000 (IHC);1:25 -1:100（Flow)；Assay Dependent（IP）;1:5,000-1:20,000(WB)

产品详细信息This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, horse, human, pig, rabbit and rat.For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10176) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and HeLa (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5 µg/mL) and detected using Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 (Product # SA5-10176) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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参考文献：

1.The Journal of clinical investigationOrphan Gpr182 suppresses ERK-mediated intestinal proliferation during regeneration and adenoma formation. 2.Journal of neurotrauma Altered Neuroinflammation and Behavior after Traumatic Brain Injury in a Mouse Model of Alzheimer's Disease.

技术参数

产品应用 Flow;ICC;IHC;IP;WB;IF

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