Rat anti-Mouse IgG2a Secondary Antibody, HRP/大鼠抗小鼠IgG2a二抗，HRP

货号：SA1-35646

规格：1 mg

价格：21153

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Immunoglobulins

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：LO-MG2a-9

荧光染料：HRP

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	Assay dependent(ELISA)；Assay dependent.(IHC (F))；1:1,000-1:10,000(WB)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG2a Secondary Antibody (SA1-35646) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-2 OS (Lane 2). The blots were probed with Anti LKB1 Mouse Monoclonal Antibody (Product # AHO1392, 2 µg/mL) and detected by chemiluminescence using Rat anti-Mouse IgG2a Secondary Antibody, HRP (Product # SA1-35646) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 55 kDa band corresponding to LKB1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), , XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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