Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800/兔抗小鼠IgG（H + L）交叉吸附二抗，DyLight 800

货号：SA5-10164

规格：500 µg

价格：2311

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG-heavy and light chain

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：DyLight 800

类型:	二抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:25 - 1:100(Flow)；1:50-1:2,000(ICC)；1:50-1:2,000(IF)；1:50-1:2,000(IHC)；Assay Dependent(IP)；1:1,000-1:20,000(WB)

产品详细信息This antibody is cross-adsorbed and exhibits minimum reactivity to human and rat.For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10164) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected using Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800 (Product # SA5-10164) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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