Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755/兔抗小鼠 IgG (H+L) 交叉吸附二抗, DyLight 755

货号：SA5-10163

规格：500μg

价格：2311

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Seconda

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:25 - 1:100(Flow)；1:50-1:2,000 (ICC)；1:50-1:2,000(IF)；1:50-1:2,000(IHC)；Assay Dependent (IP)；1:5,000-1:20,000 (WB)

产品详细信息This antibody is cross-adsorbed and exhibits minimum reactivity to human and rat.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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