CD25 Monoclonal Antibody (PC61.5), PE-Cyanine5.5, eBioscience™

货号：35-0251-80,35-0251-82

规格：25 μg,100 μg

价格：1237,3090

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：PC61.5

荧光染料：PE-Cy5.5

抗体类型:	小鼠流式抗体	同型对照：	IgG1
浓度：	0.2 mg/mL	用法：	0.25 µg/test (Flow)

Description:The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserTarget InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (35-0251-80) in FlowStaining of SJL splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG1 K Isotype Control PE-Cyanine5-5 (Product # 35-4301) (left) or 0.125 µg of Anti-Mouse CD25 PE-Cyanine5-5 (right). Total viable cells were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.PloS oneIL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes."Published figure using CD25 monoclonal antibody (Product # 35-0251-82) in Flow Cytometry"2.PloS oneNod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis."Published figure using CD25 monoclonal antibody (Product # 35-0251-82) in Flow Cytometry"3.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchHigh-dose interferon-γ promotes abortion in mice by suppressing Treg and Th17 polarization."Published figure using CD25 monoclonal antibody (Product # 35-0251-82) in Flow Cytometry"4.Cancer researchCombination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses.

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27666.htm