TRANCE (RANKL/TNFSF11) Mouse ELISA Kit/小鼠TRANCE（RANKL / TNFSF11）ELISA试剂盒

货号：EMTNFSF11,EMTNFSF11X5,EMTNFSF11X10

规格：96 tests,5X96 tests,10X96 tests

价格：7238,29891,52892

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	4 pg/mL
检测范围	2.74-2000 pg/mL
样本类型/体积	上清液 100 μL 血清 33 μL 血浆 33 μL
操作时间	1 hr 20 min
测试时间	4 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	<12%
批内变异系数（CV）	<10%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	RANKL (RANK Ligand)
Protein aliases	CD254, ODF, OPG ligand, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANKL, receptor activator of NF-kappaB ligand, receptor activator of nuclear factor kappa B ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, membrane form, soluble form, tumor necrosis factor-related activation-induced cytokine
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Ly109l, ODF, OPG, OPGL, RANKL, Tnfsf11, Trance
Gene ID	(Mouse) 21943
Gene symbol	Tnfsf11
UniProt ID	(Mouse) O35235

The Mouse TRANCE (RANKL/TNFSF11) ELISA quantitates Ms TRANCE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TRANCE.小鼠TRANCE（RANKL / TNFSF11）ELISA定量检测小鼠血清，血浆或细胞培养基中的TRANCE。 该检测试剂盒将专门识别天然和重组Ms TRANCE。Principle of the method 方法原理The Mouse TRANCE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠TRANCE固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.该基因编码肿瘤坏死因子（TNF）是细胞因子家族的成员，该家族是骨保护素的配体，并充当破骨细胞分化和激活的关键因子。该蛋白显示为树突状细胞存活因子，并参与T细胞依赖性免疫应答的调节。据报道，T细胞活化可诱导该基因表达并导致破骨细胞增多和骨质流失。该蛋白可通过涉及SRC激酶和肿瘤坏死因子受体相关因子（TRAF）6的信号复合物激活抗凋亡激酶AKT / PKB，这表明该蛋白可能在细胞凋亡的调控中起作用。小鼠相关基因的靶向破坏导致严重的骨质疏松症和破骨细胞的缺乏。缺陷小鼠在T和B淋巴细胞的早期分化中表现出缺陷，并且在怀孕期间未能形成肺泡-肺泡的乳腺结构。已经发现两个选择性剪接的转录物变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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