FLT-3L Mouse ELISA Kit /小鼠FLT-3L ELISA试剂盒

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FLT-3L Mouse ELISA Kit /小鼠FLT-3L ELISA试剂盒

货号:EMFLT3L,EMFLT3LX5,EMFLT3LX10

规格:96 tests,5X96 tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

15 pg/mL

检测范围20.48-5000 pg/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameFLT3LG
Protein aliasesFlt3 ligand, Flt3L, SL cytokine, Fms-related tyrosine kinase 3 ligand

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesFlt3l, Flt3lg, Ly72L
Gene ID(Mouse) 14256
Gene symbolFlt3l
UniProt ID

(Mouse) P49772

The Mouse Flt-3L ELISA quantitates Ms Flt-3L in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Flt-3L.小鼠Flt-3L ELISA定量检测小鼠血清,血浆或细胞培养基中的Fs-3L。 该检测试剂盒将专门识别天然和重组Ms Flt-3L。Principle of the method 方法原理The Mouse Flt-3L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠Flt-3L固相夹心ELISA(酶联免疫吸附测定)是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a Protein Coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Ras signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and cytokine activity. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.FLT3LG(Fms相关酪氨酸激酶3配体)是一种蛋白质编码基因。 与FLT3LG相关的疾病包括再生障碍性贫血和脑癌。 其相关途径包括造血干细胞分化途径和沿袭特异性标记物和Ras信号传导途径。 与该基因相关的基因本体论(GO)注释包括蛋白质同源二聚化活性和细胞因子活性。 树突状细胞(DC)通过识别病原体并引发病原体特异性免疫反应,在先天免疫和适应性免疫之间提供了关键的联系。 FLT3LG控制DC的发育,对于浆细胞样DC和CD8阳性经典DC及其CD103阳性组织对应物尤其重要。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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