| 产品介绍 | | |
| Rat anti Pig CD144 antibody, clone 123recognizes a ~120-130 kDa a single pass type I membrane protein considered to be the porcine homologue of human CD144, also known as VE-Cadherin or Cadherin-5.VE-cadherin is expressed by endothelial cells and is important in cell-cell adhesion, tight junction integrity and vascular permeabiltiy (Tinsleyet al.1999). VE-Cadherin is highly susceptible to proteolytic degradation.Western blotting and immunofluorescence using Rat anti Porcine CD144 antibody, clone 123 demonstrates rearrangement and upregulation of VE-cadherin in porcine endothelial cells cultured as part of tissue engineered blood vessels under shear stress conditions rather than static conditions (Leeet al.2012). |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Preparation | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Porcine VE-cadherin/human IgG Fc fusion protein. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Nasu, K.et al.(1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.Transplantation. 68 (6): 861-7.2.Allen, J.et al.(2008) Characterization of porcine circulating progenitor cells: toward a functional endothelium.Tissue Eng Part A. 14 (1): 183-94.3.Hoffmann, J.et al.(2008) Immobilized DNA aptamers used as potent attractors for porcine endothelial precursor cells.J Biomed Mater Res A. 84 (3): 614-21.4.Tsai, S.H.et al.(2007) Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering.Biochem Biophys Res Commun. 357 (4): 984-90.5.Zhong, A.et al.(2018) The Roles of Matrix Stiffness and β-Catenin Signaling in Endothelial-to-Mesenchymal Transition of Aortic Valve Endothelial Cells.Cardiovasc Eng Technol. 9 (2): 158-67. |
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