IL-17A Monoclonal Antibody (eBio64CAP17), PE

货号：12-7178-41,12-7178-42

规格：25 Tests,100 Tests

价格：1387,3157

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：eBio64CAP17

荧光染料：PE

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE
浓度：	5 µL/Test	用法：	Assay-Dependent (Ctrl)；Assay-Dependent(Flow)

产品详细信息Description: The eBio64CAP17 antibody reacts with human IL-17A; the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The eBio64CAP17 antibody is a neutralizing antibody. This antibody has been shown to have no reactivity to human IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). eBio64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal antibody.The eBio64CAP17 has been shown to react to rhesus and marmoset primates.Applications Reported: The eBio64CAP17 antibody has been reported for use as the capture antibody in human IL-17A ELISA and ELISPOT assays, for neutralization of IL-17A bioactivity, and for intracellular staining and flow cytometric analysis of IL-17A-producing cells.Applications Tested: This eBio64CAP17 antibody has been pre-titrated and tested by intracellular staining for flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

数据

IL-17A Antibody (12-7178-42) in FlowNormal human peripheral blood cells were stimulated overnight with PMA and Ionomycin in the presence of monensin. Cells were stained with Anti-Human CD3 FITC (Product # 11-0037-42), then fixed, permeablized, and stained with Anti-Human IL-17A PE (Left figure). The ligand blocking control demonstrates that pre-blocking Anti-Human IL-17A PE with 1.0 µg of Human IL-17A Recombinant Protein (Product # 14-8179-80) prior to staining abrogates specific IL-17A staining (Right figure). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in immunologyMonomeric Immunoglobulin A from Plasma Inhibits Human Th17 ResponsesIn VitroIndependent of FcαRI and DC-SIGN."12-7178 was used in Flow cytometry/Cell sorting to suggest the potential therapeutic utility of monomeric IgA in autoimmune and inflammatory diseases that implicate Th17 cells."AuthorsSaha C,Das M,Patil V,Stephen-Victor E,Sharma M,Wymann S,Jordi M,Vonarburg C,Kaveri SV,Bayry J2.Frontiers in immunologyIL-1β, But Not Programed Death-1 and Programed Death Ligand Pathway, Is Critical for the Human Th17 Response toMycobacterium tuberculosis."12-7178 was used in Flow cytometry/Cell sorting to investigate the role of PD pathway in regulating Th17 response to M. tuberculosis, showing that IL-1β, but not that PD-1 pathway, is critical for response."AuthorsStephen-Victor E,Sharma VK,Das M,Karnam A,Saha C,Lecerf M,Galeotti C,Kaveri SV,Bayry J3.Journal of immunology researchThe Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma."12-7178 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma."AuthorsZhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyA monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. AuthorsKap YS,van Meurs M,van Driel N,Koopman G,Melief MJ,Brok HP,Laman JD,'t Hart BA

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