IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor 660

货号：50-7219-41,50-7219-42

规格：25 Tests,100 Tests

价格：1607,3214

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：eBio3A3-N2 (3A3-N2)

荧光染料：eFluor 660

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 660
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test(Flow)

产品详细信息Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presenceof IL-15.Applications Reported: This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

数据

IL-21 Antibody (50-7219-41) in FlowIntracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Mediators of inflammationEffects of Anti-TNFαTreatment on Mucosal Expression of IL-17A, IL-21, and IL-22 and Cytokine-Producing T Cell Subsets in Crohn's Disease."Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry"2.Journal of immunology (Baltimore, Md. : 1950)Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10."50721942 was used in flow cytometry to find that oral administration of a selective Rho-associated kinase 2 inhibitor, KD025, reduces psoriasis area in 46% of patients with psoriasis vulgaris"AuthorsZanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD3.Cancer researchReprogramming CD19-specific T cells with IL-21 signaling can improve adoptive immunotherapy of B-lineage malignancies."Published figure using IL-21 monoclonal antibody (Product # 50-7219-42) in Flow Cytometry"

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