Mouse IgG Isotype Control/小鼠IgG同型对照
货号：02-6502
规格：5 mg
价格：1106
产品类型：小鼠同型对照
品牌：Thermo Fisher
抗原：Gamma Immunoglobin
物种：其它
宿主：小鼠
抗体亚型：IgG
荧光染料：其它
| 类型: | | 同型对照： | |
| 浓度： | 2.5 mg/mL | 用法： | Assay-Dependent(对照试剂（Ctrl)) |
| 产品详细信息Mouse IgG is purified by various methods from pooled normal mouse serum. The product is pure by SDS-PAGE analysis. The protein concentration is 2.5 mg/mL based on an E (1%) = 14.0 at 280 nm. 靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
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| 参考文献： |
| 1.PloS one Protective responses to sublytic complement in the retinal pigment epithelium. |
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