CD3e Monoclonal Antibody (145-2C11), FITC, eBioscience™

货号：11-0031-63,11-0031-81 ,11-0031-82 ,11-0031-85 ,11-0031-86

规格：10 μg,50 μg,100 μg,500 μg,1 mg

价格：0,511,909,2629,4661

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：145-2C11

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow);10 µg/mL(ICC);10 µg/mL(IF);10 µg/mL(IHC(F))

产品详细信息Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 145-2C11 antibody has been tested by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3e Antibody (11-0031-63) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD19 PE (Product # 12-0193-82) and 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (left) or 0.25 µg of Anti-Mouse CD3e FITC (right). Cells in the lymphocyte gate were used for analysis. CD3e Antibody (11-0031-63) in IHC (F)Immunohistochemistry of frozen mouse spleen stained with 10 µg/mL Anti-Mouse CD3e FITC and costained with 10 µg/mL Anti-Human/Mouse CD45R (B220) eFluor® 660. Nuclei are stained with DAPI. CD3e Antibody (11-0031-63)JCI insight 2016 -Published figure using CD3e monoclonal antibody (Product # 11-0031-63)

推荐产品：

▪ 流抗抗体

参考文献：

1.PloS one Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice.2.Clinical and translational allergy The efficacy of oral and subcutaneous antigen-specific immunotherapy in murine cow's milk- and peanut allergy models.3.PloS one A plant-derived VLP influenza vaccine elicits a balanced immune response even in very old mice with co-morbidities. 4.Neoplasia (New York, N.Y.) Targeting Liver Cancer and Associated Pathologies in Mice with a Mitochondrial VDAC1-Based Peptide. 5.Nature communications Extrafollicular CD4+ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

技术参数

产品应用 Flow;ICC;IHC(F);IF;TCA;IHC;IP;WB;Misc;FN

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-22244.htm