CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), FITC

货号：11-0212-81,11-0212-82,11-0212-85

规格：50μg,100μg,500μg

价格：925,1649,2892

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBio4E3 (4E3)

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The monoclonal antibody eBio4E3 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes.Applications Reported: This eBio4E3 (4E3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio4E3 (4E3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

数据

CD21/CD35 Antibody (11-0212-81) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse CD21/CD35 FITC (right). Total viable cells were used for analysis.

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参考文献：

1.Biochemical and biophysical research communicationsPostnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice."Published figure using CD21/CD35 monoclonal antibody (Product # 11-0212-82) in Immunofluorescence"2.Biochemical and biophysical research communicationsPostnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice."Published figure using CD21/CD35 monoclonal antibody (Product # 11-0212-82) in Immunofluorescence"3.Molecular medicine reportsReducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway."Published figure using CD21/CD35 monoclonal antibody (Product # 11-0212-82) in Flow Cytometry"4.Clinical immunology (Orlando, Fla.)BAFF blockade prevents anti-drug antibody formation in a mouse model of Pompe disease."11-0212 was used in Flow cytometry/Cell sorting to assess the potential of BAFF as a novel drug candidate for use in protein replacement therapies."AuthorsDoerfler PA,Nayak S,Herzog RW,Morel L,Byrne BJ5.Journal of immunology (Baltimore, Md. : 1950)Impaired apoptotic cell clearance in the germinal center by Mer-deficient tingible body macrophages leads to enhanced antibody-forming cell and germinal center responses."11-0212 was used in Flow cytometry/Cell sorting to demonstrate a critical role for Mer in GC apoptotic cell clearance by TBMΦs."AuthorsRahman ZS,Shao WH,Khan TN,Zhen Y,Cohen PL

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