CD21 Monoclonal Antibody (HB5), FITC

货号：11-0219-42

规格：100 Tests

价格：2271

产品类型：流式抗体

品牌：eBioscience

抗原：CD21

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：HB5

荧光染料：FITC

类型:	人B细胞流抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)

产品详细信息Description: The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.Applications Reported: This HB5 antibody has been reported for usein flow cytometric analysis.Applications Tested: This HB5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.

数据

CD21 Antibody (11-0219-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0038-80) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD21 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Nature communicationsPD-L1hi B cells are critical regulators of humoral immunity."11-0219 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease."AuthorsKhan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG2.European journal of immunologyLong-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers."Published figure using CD21 monoclonal antibody (Product # 11-0219-42) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion."Published figure using CD21 monoclonal antibody (Product # 11-0219-42) in Flow Cytometry"

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