CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), FITC, eBioscience™
货号：11-0083-81,11-0083-82,11-0083-85
规格：50 μg,100 μg,500 μg
价格：623,1038,2431
产品类型：流式抗体
品牌：eBioscience
抗原：CD8b
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBioH35-17.2 (H35-17.2)
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 µg/test (Flow） |
| 产品详细信息Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. |
| 数据 |
CD8b Antibody (11-0083-81) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.125 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.125 µg of Anti-Mouse CD8b FITC (right). Cells in the lymphocyte gate were used for analysis.CD8b Antibody (11-0083-81) Nature communications 2014 -Figure 5 Genetic targeting of PD-L1 expression on cancer cells reverses the CD8 + TIL dysfunction and suppresses metastasis ( a ) Cell surface expression of PD-L1 on 344SQ PD-L1 knockdown (344SQ-shPD-L1) vs 344SQ scramble control (344SQ-scr) cells by FACS (red line, isotype control staining; blue line, anti-PD-L1 staining). The measurement was independently repeated at least three times. ( b ) Primary tumor mass (top left) and lung metastases (top right) in 129/Sv mice (n = 10) injected subcutaneously with 344SQ-shPD-L1 or 344SQ-scr cancer cells. Micrometastases (bottom) observed in hematoxylin and eosin-stained lung tissue sections are indicated by yellow arrows. Scale bar, 2mm. Samples were obtained 6 weeks post-injection. The data from two independent experiments were pooled. Data are shown as mean +- s.e.m. t -test was used to analyze. P values are shown in the graphs. ( c , d ) FACS analysis of ( c ) surface PD1, LAG3, and TIM3 marker expression levels on CD8 + T cells; ( d ) CD8 + TIL frequency for primary tumors in 129/Sv mice (n = 5) injected subcutaneously with 344SQ-shPD-L1 (shPD-L1) or 344SQ-scr control (Control) cancer cells and necropsied 2 weeks later. The analyses were independently repeated three times. Data are shown as mean +- s.e.m. t -test was used to analyze. P values are shown in the graphs. |
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| 参考文献： |
| 1.Nature communications Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.2.PloS one Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.3.Oncotarget Tumor sialylation impedes T cell mediated anti-tumor responses while promoting tumor associated-regulatory T cells.4.Oncoimmunology Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity.5.The Journal of biological chemistry Granulocyte/Macrophage Colony-stimulating Factor-dependent Dendritic Cells Restrain Lean Adipose Tissue Expansion. |
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