CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™/CD49d (Integrin alpha 4) 单克隆(R1-2), 功能抗体, eBioscience™

货号：16-0492-82,16-0492-85,16-0492-38,16-0492-025,16-0492-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1992,3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD49d

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：R1-2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（对照）;Assay-Dependent（功能检测）

产品详细信息Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis. R1-2 has also been reported in in vitro functional assays.Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.仅用于科研。不用于诊断过程。

数据

CD49d (Integrin alpha 4) Antibody (16-0492-82) in FlowMouse splenocytes were stained with 0.25 µg of Rat IgG2b kappa Isotype Control (Product # 14-4031-85) (blue histogram) or 0.25 µg of CD49d Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2b Monoclonal Antibody, PE (Product # 12-4815-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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参考文献：

流式1.Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry"2.OncotargetThe impact of ranitidine on monocyte responses in the context of solid tumors."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry"3.Frontiers in immunologyInterleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry"中和4.Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration."16-0492 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration."AuthorsBrinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS功能检测5. Molecular therapy. Methods and clinical developmentSuppression of leaky expression of adenovirus genes by insertion of microRNA-targeted sequences in the replication-incompetent adenovirus vector genome."16-0492 was used in Immunocytochemistry to investigate how insertion of microRNA-targeted sequences into replication-incompetent adenovirus vector genomes can suppress leaky expression of adenoviral genes."AuthorsShimizu K,Sakurai F,Tomita K,Nagamoto Y,Nakamura S,Katayama K,Tachibana M,Kawabata K,Mizuguchi H6. StrokeBlocking of α4 integrin does not protect from acute ischemic stroke in mice."16-0492 was used in Functional assays to cast doubt on the effectiveness of anti-CD49d as a stroke treatment."AuthorsLanghauser F,Kraft P,Göb E,Leinweber J,Schuhmann MK,Lorenz K,Gelderblom M,Bittner S,Meuth SG,Wiendl H,Magnus T,Kleinschnitz C

技术参数

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