CD24 Monoclonal Antibody (30-F1), FITC
货号：11-0241-82,11-0241-85
规格：100 μg,500μg
价格：1280,1280
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：大鼠
抗体亚型：其它
克隆号：30-F1
荧光染料：FITC
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1 μg/test(Flow) |
产品详细信息Description: The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylatedmolecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations.Applications Reported: The 30-F1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, andcontrol of autoimmunity.
| 数据 |
| CD24 Antibody (11-0241-81) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0191-82) and 0.5 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or 0.5 µg of Anti-Mouse CD24 FITC (right). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Development (Cambridge, England)Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression ofShh."Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry"2.Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry"3.Cell reportsLineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors."11-0241 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage."AuthorsBoulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N4.The Journal of investigative dermatologyβ-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the ReticularDermis."11-0241 was used in Flow cytometry/Cell sorting to study how Wnt/β-catenin signalling may control adipogenic cell fate within the lower dermis, which potentially contributes to the pathogenesis of fibrotic skin diseases."AuthorsMastrogiannaki M,Lichtenberger BM,Reimer A,Collins CA,Driskell RR,Watt FM5.Cell cycle (Georgetown, Tex.)Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer."11-0241 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning."AuthorsQin Y,Qin J,Zhou C,Li J,Gao WQ |
QQ:1749072012