CD25 Monoclonal Antibody (CD25-4E3), FITC

货号：11-0257-41,11-0257-42

规格：25 Tests,100 Tests

价格：0,2323

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：CD25-4E3

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), FITC
浓度：	5 µL/Test	用法：	5 μL (0.25 μg)/test(Flow)

产品详细信息Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.This monoclonal antibody is cross-reactive to rhesus monkey.Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

数据

CD25 Antibody (11-0257-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (left) or Anti-Human/Non-Human Primate CD25 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in immunologyLenalidomide and Programmed Death-1 Blockade Synergistically Enhances the Effects of Dendritic Cell Vaccination in a Model of Murine Myeloma."11-0257 was used in Flow cytometry/Cell sorting to investigate the efficacy of dendritic cell vaccination in combination with lenalidomide and PD-1 blockade in a model of murine myeloma."AuthorsVo MC,Jung SH,Chu TH,Lee HJ,Lakshmi TJ,Park HS,Kim HJ,Rhee JH,Lee JJ2.OncoimmunologyHLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients."Published figure using CD25 monoclonal antibody (Product # 11-0257-42) in Flow Cytometry"3.International journal of molecular medicineTIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts."11-0257 was used in Flow cytometry/Cell sorting to indicate that blocking TIM-4 expression by Kupffer cells via exogenous TGF-β may be a strategy to inhibit rejection of liver allografts."AuthorsWu H,Xu X,Li J,Gong J,Li M4.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer."Published figure using CD25 monoclonal antibody (Product # 11-0257-42) in Flow Cytometry"5.Cancer immunology, immunotherapy : CIITumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1."Published figure using CD25 monoclonal antibody (Product # 11-0257-42) in Flow Cytometry"

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