CD25 Monoclonal Antibody (P4A10), FITC

货号：11-0250-41,11-0250-42

规格：25 Tests,100 Tests

价格：1629,2580

产品类型：流式抗体

品牌：eBioscience

物种：其它

宿主：小鼠

抗体亚型：IgG

克隆号：P4A10

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)

产品详细信息Description: This P4A10 monoclonal antibody reacts with canine CD25, the 55-kDa interleukin-2 receptor alpha (IL-2Ralpha) chain. As in humans and mice, CD25 in dogs is expressed by early T and B lineage progenitors as well as by activated mature T and B lymphocytes. CD25 associates with the IL-2R beta (CD122) and gamma (CD132) chains to form the high-affinity IL-2 receptor. On its own, CD25 binds IL-2 with low affinity. Along with CD4 and Foxp3, CD25 is a major marker used to define regulatory Tcells.The P4A10 antibody has been reported to recognize the same epitope as the anti-human CD25 monoclonal antibody ACT-1, but with greater affinity.Applications Reported: This P4A10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P4A10 antibody has been pre-titrated and tested by flow cytometry on normal canine peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

数据

CD25 Antibody (11-0250-41) in FlowStaining of normal canine peripheral blood cells with Anti-Canine CD4 APC (Product # 17-5040-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Canine CD25 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Molecular therapy : the journal of the American Society of Gene TherapyDonor bone marrow-derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu."11-0250 was used in Flow cytometry/Cell sorting to describe the generation of BMDCs and glucocorticoid-treated BMDCs with potent immunomodulatory properties for application in allogeneic transplantation."AuthorsO'Flynn L,Treacy O,Ryan AE,Morcos M,Cregg M,Gerlach J,Joshi L,Nosov M,Ritter T2.Veterinary immunology and immunopathologyCanine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells. AuthorsBismarck D,Schütze N,Moore P,Büttner M,Alber G,Buttlar Hv3.PloS oneFunctional glycosylation of dystroglycan is crucial for thymocyte development in the mouse."Published figure using CD25 monoclonal antibody (Product # 11-0250-42) in Flow Cytometry

技术参数

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