CD14 Monoclonal Antibody (61D3), FITC, eBioscience™

货号：11-0149-41,11-0149-42

规格：25 tests,100 tests

价格：941,2139

产品类型：流式抗体

品牌：eBioscience

抗原：CD14

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：61D3

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test (Flow)

产品详细信息Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

数据

CD14 Antibody (11-0149-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD14 FITC (purple histogram). Cells in the monocyte gate were used for analysis. CD14 Antibody (11-0149-41)Stem cell research and therapy 2017 - Fig. 1 Flow cytometry analysis of phenotype characterization of hUCMSCs. Phenotype of CD73, CD90, CD105, CD14, CD34, CD45, CD79a and HLA-DR of hUCMSCs was detected by flow cytometry. Intensity >= 95% represented strong expression while <=2% represented low or no expression CD14 Antibody (11-0149-41)Retrovirology 2017 -Fig. 1 Isolation and characterization of huglia/HIV (HC69) cells. a Schematic representation of a typical procedure to develop a microglia/HIV clonal cell population such as huglia/HIV (HC69) cells. Uninfected clonal populations are indicated in grey boxes , and latently-infected clonal populations are indicated in blue boxes . b Immunofluorescence analysis of the human microglial cells huglia/HIV (HC01). Cells were cultured, fixed, and immunostained with either anti-CD11b ( green ), anti-CD14 ( red ) or anti-P2RY12 ( red ) conjugated antibodies. Nuclei were stained with DAPI ( blue ). Merged images of nuclei, CD11b and CD14, or nuclei, CD11b and P2RY12 are indicated

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参考文献：

1. Oncoimmunology Prostaglandin E2-mediated adenosinergic effects on CD14+ cells: Self-amplifying immunosuppression in cancer.2. Frontiers in immunology Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model.3. Nature communications Cellular metabolism constrains innate immune responses in early human ontogeny.4. Retrovirology Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells.5. Scientific reports Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

技术参数

产品应用 Flow;ICC;IF;Misc

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