CD45RB Monoclonal Antibody (C363.16A), FITC

货号：11-0455-81,11-0455-82,11-0455-85

规格：50μg,100μg,500μg

价格：511,909,2738

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：C363.16A

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

数据

CD45RB Antibody (11-0455-81) in FlowStaining of BALB/c splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (open histogram) or 0.25 µg of Anti-Mouse CD45RB FITC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.JCI insightAdjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice againstToxoplasma gondii."11-0455 was used in Flow cytometry/Cell sorting to develop multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant to protect against Toxoplasma gondii."AuthorsEl Bissati K,Chentoufi AA,Krishack PA,Zhou Y,Woods S,Dubey JP,Vang L,Lykins J,Broderick KE,Mui E,Suzuki Y,Sa Q,Bi S,Cardona N,Verma SK,Frazeck L,Reardon CA,Sidney J,Alexander J,Sette A,Vedvick T,Fox C,Guderian JA,Reed S,Roberts CW,McLeod R2.European journal of immunologyIL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8(+) T cells."11-0455 was used in Flow cytometry/Cell sorting to study the upregulation of CD39 on CD8(+) T cells and identify a novel pathway of peripheral tolerance."AuthorsNoble A,Mehta H,Lovell A,Papaioannou E,Fairbanks L3.Journal of neuroinflammationSTAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE."11-0455 was used in Flow cytometry/Cell sorting to establish the role of STAT4 in the regulation of cytokine production during experimental autoimmune encephalomyelitis pathogenesis."AuthorsMcWilliams IL,Rajbhandari R,Nozell S,Benveniste E,Harrington LE4.Journal of immunology (Baltimore, Md. : 1950)Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses."11-0455 was used in Flow cytometry/Cell sorting to investigate whether Leishmania braziliensis infection efficiently triggers innate responses in dendritic cells and the subsequent adaptive immune responses for parasite clearance."AuthorsVargas-Inchaustegui DA,Xin L,Soong L5.Infection and immunityEffects of CXCL10 on dendrtic cell and CD4+ T-cell functions during Leishmania amazonensis infection."11-0455 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying enhanced immunity against L. amazonensis infection following CXC10 treatment, showing that treatment has an effect on dendritic cell and CD4+ T-cell functions."AuthorsVasquez RE,Xin L,Soong L

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