CD45RO Monoclonal Antibody (UCHL1), FITC

货号：11-0457-42

规格：100 Tests

价格：753

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：UCHL1

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC
浓度：	5 µL/Test	用法：	5 μL (0.5 μg)/test(Flow)

产品详细信息Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHL1 antibody has been pre-titrated and testedby flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

数据

CD45RO Antibody (11-0457-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Anti-Human CD45RO FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in cellular and infection microbiologyTh2/1 Hybrid Cells Occurring in Murine and Human Strongyloidiasis Share Effector Functions of Th1 Cells."11-0457 was used in Flow cytometry/Cell sorting to assess the T helper cell phenotypes in threadworm-infected patients and experimental murine infections with focus on CD4+ T cells co-expressing markers of Th2 and Th1 differentiation."AuthorsBock CN,Babu S,Breloer M,Rajamanickam A,Boothra Y,Brunn ML,Kühl AA,Merle R,Löhning M,Hartmann S,Rausch S2.Oncology lettersA different representation of natural T cells and natural killer cells between tumor-infiltrating and periphery lymphocytes in human hepatocellular carcinoma."11-0457 was used in Flow cytometry/Cell sorting to describe natural T cells and NK cells in local tumour tissues and the periphery blood of patients with human hepatocellular carcinoma."AuthorsLi XF,Dai D,Song XY,Liu JJ,Zhu L,Zhu X,Ma W,Xu W3.Cell researchBlocking the recruitment of naive CD4+T cells reverses immunosuppression in breast cancer."Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry"4.Cancer biology and therapyDurable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy."11-0457 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy."AuthorsWyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ5.OncoimmunologyFunctional expression of CD137 (4-1BB) on T helper follicular cells."Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry"

技术参数

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