CD45R (B220) Monoclonal Antibody (HIS24), FITC

货号：11-0460-82

规格：100μg

价格：1518

产品类型：流式抗体

品牌：eBioscience

物种：大鼠

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HIS24

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), FITC
浓度：	0.5 mg/mL	用法：	1 µg/test(Flow)；10 µg/mL(ICC)；10 µg/mL(IF)；10 µg/mL(IHC (F))

产品详细信息Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS24 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

数据

CD45R (B220) Antibody (11-0460-82) in FlowWistar rat splenocytes were stained with 0.5 µg of Mouse IgG2b kappa Isotype Control, FITC (Product # 11-4732-82) (blue histogram) or 0.5 µg of CD45R Monoclonal Antibody, FITC (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

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参考文献：

1.Cell reportsMll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development."11-0460 was used in Flow cytometry/Cell sorting to elucidate the contribution of MII-AF4 in B cell acute lymphoblastic leukaemia early development in the mouse embryo."AuthorsBarrett NA,Malouf C,Kapeni C,Bacon WA,Giotopoulos G,Jacobsen SEW,Huntly BJ,Ottersbach K2.Journal of virologyCytomegalovirus CC chemokine promotes immune cell migration."11-0460 was used in Flow cytometry/Cell sorting to indicate that rat cytomegalovirus r129 is highly chemotactic, leading to implications for infection, reactivation and acceleration of transplant vascular sclerosis."AuthorsVomaske J,Denton M,Kreklywich C,Andoh T,Osborn JM,Chen D,Messaoudi I,Orloff SL,Streblow DN

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