CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC

2024-10-18

CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC

货号:11-0519-41,11-0519-42

规格:25 Tests,100 Tests

价格:822,1855

产品类型:流式抗体

品牌:eBioscience

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:23C6

荧光染料:FITC

类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度:5 µL/Test用法:5 μL (1 μg)/test(Flow)
产品详细信息Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 23C6 antibody has been pre-titrated and tested by flow cytometric analysis of human melanoma cell line and peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
数据
CD51/CD61 (Integrin alpha v beta 3) Antibody (11-0519-41) in FlowStaining of the M21 cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD51/CD61 (Integrin alpha/beta 3) FITC (filled histogram). Total viable cells were used for analysis.
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参考文献:

1.Stem cells (Dayton, Ohio)Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

AuthorsMenon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J2.Molecular therapy. Nucleic acidsAn Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma InVitro and InVivo."11-0519 was used in Immunocytochemistry-immunoflourescence to reveal a great translational potential of biRGD-siPIK3CB conjugate as a novel molecule for glioblastoma therapy."AuthorsCen B,Wei Y,Huang W,Teng M,He S,Li J,Wang W,He G,Bai X,Liu X,Yuan Y,Pan X,Ji A3.ImmunologyInterferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus."11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus."AuthorsSwindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD4.Human reproduction (Oxford, England)Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro."11-0519 was used in Flow cytometry/Cell sorting to study an increase in apical fibronectin in blastocysts mediated by insulin-like growth factor to increase blastocyst attachment to endometrial epithelial cells in vitro."5.Nucleic acids researchTumor-targeted in vivo gene silencing via systemic delivery of cRGD-conjugated siRNA."11-0519 was used in Flow cytometry/Cell sorting to assess the anti-tumour potential of an siRNA molecule targetting VEGFR2 conjugated to cRGD peptide."AuthorsLiu X,Wang W,Samarsky D,Liu L,Xu Q,Zhang W,Zhu G,Wu P,Zuo X,Deng H,Zhang J,Wu Z,Chen X,Zhao L,Qiu Z,Zhang Z,Zeng Q,Yang W,Zhang B,Ji A
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