CD62L (L-Selectin) Monoclonal Antibody (SK11), FITC

货号：11-0625-42

规格：100 Tests

价格：2271

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：SK11

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)

产品详细信息Description: This SK11 monoclonal antibody reacts with human and non-human primate CD62L (also known as L-selectin and LECAM). CD62L is expressed on neutrophils, monocytes, and subsets of T, B, and NK cells, as well as endothelial cells. This cell surface adhesion molecule binds glycolsylated, fucosylated and sulfated sialylated glycoproteins, including CD34, glycam-1, and MadCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homingof cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.The SK11 antibody has been reported to recognize chimpanzee, rhesus, cynomolgus monkey, olive baboon, and pigtailed macaque as well as pig.Applications Reported: This SK11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

数据

CD62L (L-Selectin) Antibody (11-0625-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human/NHP CD62L (L-selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.

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