Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC

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Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC

货号:11-0719-41,11-0719-42

规格:25 Tests,100 Tests

价格:1029,2167

产品类型:流式抗体

品牌:eBioscience

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:OKT9 (OKT-9)

荧光染料:FITC

类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度:5 µL/Test用法:5 µL (0.125 µg)/test(Flow);0.125 µg/mL (ICC);0.125 µg/mL (IF)
产品详细信息Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means ofdetecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
数据
CD71 (Transferrin Receptor) Antibody (11-0719-41) in FlowStaining of Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0037-81 and Product # 16-0289-81)-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD71 (Transferrin Receptor) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:

1.Blood advancesMechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation."11-0719 was used in Flow cytometry/Cell sorting to study the mechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation."AuthorsBartholdy B,Lajugie J,Yan Z,Zhang S,Mukhopadhyay R,Greally JM,Suzuki M,Bouhassira EE2.Nature communicationsGenetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin."Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry"3.PloS oneKnockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells."11071942 was used in flow cytometry to determine that HSPA9 knockdown is associated with increased TP53 expression and activity"AuthorsLiu T,Krysiak K,Shirai CL,Kim S,Shao J,Ndonwi M,Walter MJ4.CellFunctional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation."11-0719 was used in Flow cytometry/Cell sorting to demonstrate how variation in a single cytokine can lead to biased downstream signaling and can thereby cause human disease."AuthorsKim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG5.Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells."11-0719 was used in Flow cytometry/Cell sorting to investigate whether activation of exogenous HOXB4 promotes the production of more mature, definitive red blood cells."
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