POROS™ HQ 50 µm Strong Anion Exchange Resin/POROS HQ 50 µm强阴离子交换树脂
货号：82077,82078
规格：10 mL,50 mL
价格：2171,7252
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| POROS™ 50-micron resins are available for low pressure applications, including large scale downstream bioprocessing. POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography™ resin designed for the separation and purification of biomolecules.POROS Perfusion Chromatography resinsA high performance chromatography resin for analytical to process scale separations.• Higher productivity—high throughput and high dynamic capacity associated with Perfusion Chromatography• Chemical stability—allows aggressive cleaning and sanitization• Enhanced biomolecule access—provided via large pores, ranging between 500-10000 Å• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix• Develop better separations methods in a shorter time frame—the speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separationReduce time-consuming sample prepYou can replace many sample preparation steps with high-speed Perfusion Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.Create novel assays for more efficient analysisPerfusion Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.High capacity, high resolution, high speedIn contrast to conventional chromatography media, POROS Perfusion Chromatography resin particles are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased—without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm/hr compared to 50 to 360 cm/hr for conventional resins. |
| 数据： |
Dynamic binding capacity over a range of operating flow ratesPOROS 50 HQ and XQ provide high dynamic binding capacities and excellent capture efficiencies at higher linear velocities. Dynamic binding capacity was determined by loading a 10 mg/mL BSA solution onto a 3.3 mL until a 5% breakthrough was reached. The experiment was then repeated using different flow rates. Experimental conditions: Column: 0.46 cmD x 20 cmL; Load condition: 10 mg/mL BSA in 20 mM Tris, pH 8.0 Resolution over a range of operating flow ratesPOROS 50 HQ is capable of achieving excellent seperation using a very steep gradient and maintaining resolution at multiple flow rates. A protein mixure of holo-transferrin, ovalbumin, and soybean trypsin inhibitor was loaded onto a 3.3 mL column and then eluted using a 0-50% B gradient over 10 column volumns. The experiment was then repeated using different flow rates. Experimental conditions: Column: 0.46 cmD x 20 cmL, Buffer A: 20 mM Tris, pH 8.0, Buffer B: 20 mM Tris, 1.0 M NaCl, pH 8.0. |
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