Mouse anti Human Interleukin-4 Receptor antibody, clone 25463
货号：0100-0462
规格：0.1 mg
价格：4888
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：人
宿主：小鼠
抗体亚型：IgG2a
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human Interleukin-4 Receptor antibody, clone 25463recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kDa type I single pass transmembrane glycoprotein bearing a singlefibronectin type IIIdomain. Interleukin-4 Receptor form a heterodimeric complex with thecytokine receptor common subunit gamma, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.5% Trehalose |
| Immunoge | Spodoptera frugiperdalepidopteran ovarian cell lineSf21-derived, recombinant human interleukin 4 receptor, extracellular domain. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20°C only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/250 | 1/1000 |
| Flow Cytometry | √ | 1/10 | 1/40 |
| Immunohistology - Paraffin | √ | 1/20 | 1/60 |
| Western Blotting | √ | 1/125 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
技术参数 产品应用 Elisa
产品类型 Monoclonal Antibody
推荐用于 Elisa
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