Histone H4 Polyclonal Antibody/组蛋白H4多克隆抗体

货号：720166

规格：100 µg

价格：0

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to Human HIST1H4A (aa 1-126)

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.This antibody is predicted to react with Mouse, Monkey and Dog.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

Histone H4 Antibody (720166) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of Histone H4 using Histone H4 Rabbit Polyclonal antibody (Product # 720166, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Histone H4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H4. Panel e) represents control cells with no primary antibody to assess background. Histone H4 Antibody (720166) in ChIPEnrichment of endogenous Histone H4 protein using Anti- Histone H4 Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti- Histone H4 Rabbit Polyclonal Antibody (Product # 720166, 3 µg) on sheared chromatin from 2 million Jurkat cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active cFOS, B-Actin genes, region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. PLoS pathogensThe KAT5-Acetyl-Histone4-Brd4 axis silences HIV-1 transcription and promotes viral latency.

技术参数

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