CD29 (Integrin beta 1) Monoclonal Antibody (KMI6), eBioscience™

货号：14-0292-81,14-0292-82,14-0292-85

规格：50µg,100µg,500µg

价格：1340,1334,2767

产品类型：流式抗体

品牌：eBioscience

抗原：CD29

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：KMI6

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.125µg/test(Flow）;2 µg/mL(WB）

Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alpha beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin.Applications Reported: This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions.Applications Tested: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD29 (Integrin beta 1) Antibody (14-0292-81) in WB Balb/c spleen cell lysate (3x10e5 cells/lane) was resolved by SDS-PAGE under non-reducing conditions, immunoblotted with 2 µg/mL Anti-Mouse CD29 Purified and revealed with Anti-Rat IgG HRP.

推荐产品：

间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD29流式抗体细胞因子

参考文献：

1.Nature communicationsThe matricellular protein CCN1 mediates neutrophil efferocytosis in cutaneous wound healing."14-0292 was used in Blocking experiments to establish the matricellular protein, CCN1, as a critical opsonin in skin injury."Authors Jun JI,Kim KH,Lau LF

技术参数

产品应用 Flow；IP；FN; WB；IHC (F)

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16790.htm