H3K64me3 Polyclonal Antibody/H3K64me3多克隆抗体

货号：PA5-40099

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.93 mg/mL	用法：	1-2 µg(ChIP)；1:5,500(ELISA)；1:1000(WB)；1:5000(Array)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K64me3 Antibody (PA5-40099) in WBWestern Blot was performed on histone extracts (30 µg) from HeLa cells using a Tri-Methyl-Histone H3 (Lys64) polyclonal antibody (Product # PA5-40099) at a dilution of 1:100 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left, the position of the protein is indicated on the right. H3K64me3 Antibody (PA5-40099) in ChIPChIP assays were performed on human K562 cells using a Tri-Methyl-Histone H3 (Lys64) polyclonal antibody (Product # PA5-40099) and optimized PCR primer sets for qPCR. ChIP was performed with sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analyzed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and Sata satellite repeats, used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

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