Allopregnanolone ELISA Kit/异戊烷醇 ELISA试剂盒
货号：EIAALLO,EIAALLOX10
规格：96 Tests,10X96 tests
价格：7467,57271
产品类型：ELISA检测
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 129.7 pg/mL |
| 检测范围 | 156.25-10,000 pg/mL |
样本类型/体积 | 血清 50 µL 血浆 50 µL 粪便提取物 50 µL 尿液 12.5 µL 上清液 50 µL |
操作时间 | 45 min |
测试时间 | 16 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 8% |
| 批内变异系数（CV） | 7% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 抗-lgG包被96孔板 - 标准品 - 测定稀释浓缩液 - 捕获抗体 - HRP结合抗原 - 洗涤浓缩液 - TMB底物 - 终止液 - 胶板盖 |
储存温度 | -20°C |
| Protein name | 20-Hydroxyecdysone |
物种（Tested） | 人 |
试剂盒类型 | Competitive ELISA |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| The Allopregnanolone Competitive ELISA quantitates allopregnanolone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure allopregnanolone independent of species. A allopregnanolone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit allopregnanolone antibodies and allopregnanolone-peroxidase conjugate is added to the wells. The allopregnanolone-peroxidase conjugate and any allopregnanolone in the sample will compete to bind to the anti-rabbit allopregnanolone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of allopregnanolone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Allopregnanolone竞争性ELISA定量分析血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的allopregnanolone。|方法原理：孕酮代谢物竞争性ELISA研究专用试剂盒旨在定量测量与物种无关的allopregnanolone。提供了去甲萘烷酮标准品以生成测定的标准曲线，并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔同种异戊烯醇酮抗体和同种异戊烯醇酮-过氧化物酶偶联物添加到孔中。样品中的Allopregnanolone-过氧化物酶结合物和任何allopregnanolone将竞争结合抗兔Allopregnanolone抗体。温育后，洗涤板并加入底物。底物与结合的Allopregnanolone-过氧化物酶结合物反应。孵育30分钟后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色的强度与样品中存在的异戊四烯酮的含量成反比。|严格的验证：对该ELISA试剂盒的每个制造批次都进行了质量检测，测试标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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